Dr Marius Wernig, Professor at Stanford University and Co-director of the Institute of Stem Cell Biology and Regenerative Medicine, has paved the way for the application of induced pluripotent stem cells (iPSCs) to cure Epidermolysis Bullosa (EB). StemFit® culture medium, manufactured by Ajinomoto and available globally from AMSBIO has been a staple in Dr Wernig’s lab from early on in his research, providing a reliable medium to maintain normal karyotype and pluripotency of the iPSCs.
Cell surface levels of Heparan Sulfate preserved using CELLBANKER 2
September 27, 2022
How to be GMP Ready: A Guide
August 31, 2022Summary of key findings
- Use of iPSCs reduces risk of cancer and improves scalability
- Reprogramming and gene editing steps have been enhanced by Dr Wernig and team concurrently with development of differentiation and grafting steps by Prof. Anthony Oro and Colleagues (also at Stanford Medicine)
- Procedure and efficiency have been optimized using CRISPR
- Future research aims to meet regulatory challenges by replacing the entire locus associated with EB
To provide an update and first-hand account of this exciting research, we invited Dr. Wernig to an interview covering topics from inception through to future challenges. Click the link to read below!
AMSBIO's Stem Cell Synergy Solutions
StemFit® is a range of xeno-free, chemically defined media proven to effectively maintain iPSCs and embryonic stem cells (ESCs) under feeder-free conditions during the reprogramming, expansion and differentiation phases of stem cell culture and growth medium. Available in research grade and GMP formulations, StemFit® combines market-leading colony forming efficiency with lower than standard media volume-consumption to offer the most cost-effective colony expansion compared to leading competitors.
AMSBIO is the global source of StemFit® media, as part of our Stem Cell Synergy, bringing together:
- StemFit culture media, differentiation supplement and Purotein recombinant proteins
- iMatrix recombinant ECM, using E8 fragments to get more integrin binding for less protein
- CELLBANKER and STEM-CELLBANKER freezing media.
Further reading
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Neumayer, G., Torkelson, J.L., Li, S., McCarthy, K., Zhen, H.H., Madhuri Vangipuram, Mader, M.M., Gulilat Gebeyehu, Jaouni, T.M., Jacków-Malinowska, J., Rami, A., Hansen, C., Guo, Z., Gaddam, S., Tate, K.M., Pappalardo, A., Li, L., Chow, G.M., Roy, K.R. and Thuylinh Michelle Nguyen (2024). A scalable and cGMP-compatible autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa. Nature Communications, 15(1). Available online: https://doi.org/10.1038/s41467-024-49400-z
- For further information on StemFit stem cell culture media, please visit: https://www.amsbio.com/stemfit-stem-cell-culture/ or contact AMSBIO on+44-1235-828200 / +1-617-945-5033 / [email protected].
