Fig.1. Staining images of ACE2-expressing iPS cells grown in StemFit® medium with iMatrix-511 as ECM after cryopreservation in STEM CELLBANKER® infected with SARS-CoV-2 virus. Green: SARS-CoV-2 N protein, Red: OCT3/4, Blue: DAPI. Image courtesy of Kazuo Takayama (CiRA, Kyoto University, Japan)
iMatrix Recombinant Laminin E8 Fragments
Efficient cell culture and differentiation of ES/iPS cells
Laminin is a type of extracellular matrix protein that functions as an adhesion molecule for various cells in the body. This heterotrimeric molecule - with alpha, beta and gamma chains - interacts with cell membrane receptor integrins via the E8 region, which is the smallest integrin-binding component. It is the alpha chain that mainly determines the function of the laminin, particularly how it controls cell behaviour and cell fate. Thus, the type of laminin present changes during the differentiation process of cells. By utilising the combination of laminin and cells in vitro for cell culture, it is possible to efficiently induce differentiation of pluripotent stem cells.
AMSBIO offers the recombinant laminin E8 fragments of laminin-511, laminin-411, laminin-221, laminin-332 and laminin-111.
iMatrix can also be used together with our StemFit cell culture media and CELLBANKER series cryopreservation solution, to create the perfect Stem Cell Synergy for your ES/iPSC cell culture!
Real World Application
Dr Kazuo Takayama (CiRA, Kyoto University) has successfully modelled the SARS-CoV-2 life cycle in undifferentiated iPSCs, which were previously uninfectable using iMatrix, StemFit and STEM-CELLBANKER. You can find the on demand recording of the talk in our blog.
iMatrix Series
The iMatrix series can be used for differentiation of iPSCs into ectoderm, mesoderm and endoderm:
iMatrix-511 (α5β1γ1)– maintenance and expansion culture of pluripotent stem cells
iMatrix-411 (α4β1γ1) – induction of differentiation of vascular endothelial cells from ES/iPS cells
iMatrix-221 (α2β2γ1)– purification/maintenance culture of cardiomyocytes/skeletal muscle cells
iMatrix-332 (α3β3γ2)– induction of differentiation from iPS cells to corneal epithelial cells
iMatrix-111 (α1β1γ1)– induction of differentiation from human iPS cells to hepatoblast-like cells
Laminin and Integrin Interactions
iMatrix Palette
All the iMatrix series in box
1 vial x iMatrix-111, 1 vial x iMatrix-221, 1 vial x iMatrix-332, 1 vial x iMatrix-411, 1 vial x iMatrix-511, and 1 vial x iMatrix-511 silk
- For preparing suitable substrates for each cell type in microphysiological systems (MPS)
- For replicating the in vivo environment of cells in cell culture
- For exploring substrates for the differentiation of pluripotent stem cells into targeted cells
- For exploring substrates for primary cell culture
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iMatrix-511 Recombinant Laminin Series
Amsbio’s iMatrix-511 series of laminin products easily enables extended culture of human pluripotent stem cells and has swiftly established itself as the gold standard for stem cell culture.
iMatrix-411
For induction of vascular endothelial cells from hPSCs
Laminin-411 (α4β1γ1) is the major isoform that lines the basement membrane of blood vessels. It is thought to be involved in maintaining vascular homeostasis by binding to the integrin α6β1 protein on the cell surface of vascular endothelial cells. In addition, it is also known to adhere to leukocytes and platelets, important for the immune system.
iMatrix-411 contains the essential laminin-411 E8 fragments which retains full integrin binding activity but lacks binding activity to other cell matrix components. iMatrix-411 can be used to efficiently induce vascular endothelial cells and bile duct epithelial cells from hPSCs/iPSCs by binding to integrin α6β1.
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iMatrix-221
For enrichment and maintenance of cardiomyocytes
Laminin 221 (α2β2γ1) is abundantly present in the basement membrane of muscle tissues such as cardiac muscle and skeletal muscle. It binds to the integrin α7X2β1 protein, which is selectively expressed in muscle tissue. It is thought to be involved in the differentiation and functional maintenance of muscle cells.
iMatrix-221 is recombinant laminin-221 E8 fragment and exhibits high adhesive activity and selectivity as a culture medium for cardiomyocytes and skeletal muscle cells.
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iMatrix-332
Induction of differentiation of vascular endothelial cells from ES/iPS cells
Laminin-332 (α3β3γ2) is present in keratinocytes and cornea and is known to bind to integrin α3β1 and α6β4. It is also essential for epithelial cell adhesion to the basement membrane.
iMatrix-332 is the highly purified E8 region (including the integrin binding site) of human laminin 332.
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iMatrix-111
Induction of differentiation from human iPS cells to hepatoblast-like cells.
Laminin-111 (α1β1γ1) is a major substrate for nerve axon growth, both in vivo and in vitro. It's also the main component of the industry standard substrates used in cell culture: Matrigel and Cultrex BME. Laminin-111 is known to be present in the liver and bind to integrin α7X2β1 and α6β1 proteins, and is thought to be involved in the maintenance of liver tissue function.
Our iMatrix-111 is the highly purified E8 region (including the integrin binding site) of human laminin-111 and has been used for the differentiation of iPSCs into hepatocyte-like cells.
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Sanaki-Matsumiya, M., Matsuda, M., Gritti, N., Nakaki, F., Sharpe, J., Trivedi, V., & Ebisuya, M. (2022). Periodic formation of epithelial somites from human pluripotent stem cells. Nature Communications,13(1), 1-14.
Cites iMatrix-511 silk and StemFit® Basic04 CT culture medium; also Lipidure-CM5206 for low adhesion culture
Tanosaki, S., Akiyama, T., Kanaami, S., Fujita, J., Ko, M., Fukuda, K., & Tohyama, S. (2022). Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis. STAR Protocols, 3(2), 101360.
Cites iMatrix-511, iMatrix-221 and StemFit® Basic03 culture medium
Kihara, Y., Homma, J., Takagi, R., Ishigaki, K., Nagata, S., & Yamato, M. (2022). Laminin-221-derived recombinant fragment facilitates isolation of cultured skeletal myoblasts. Regenerative Therapy, 20, 147-156.
Cites iMatrix-221
Hwang, Y. S., Suzuki, S., Seita, Y., Ito, J., Sakata, Y., Aso, H., ... & Sasaki, K. (2020). Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells. Nature Communications, 11(1), 1-17.
Cites iMatrix-511 Silk with StemFit® Basic04 medium, with cell collection in CELLOTION and cryopreservation in CELLBANKER I
Aoki, H., Yamashita, M., Hashita, T., Iwao, T., & Matsunaga, T. (2020). Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity. Fluids And Barriers Of The CNS, 17(1).
Cites iMatrix-221, iMatrix-411 and iMatrix-511
Hayashi, R., Ishikawa, Y., Katori, R., Sasamoto, Y., Taniwaki, Y., & Takayanagi, H., ... & Nishida, K. (2017). Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nature Protcols, 12(4), 683-696.
Cites iMatrix-511 with StemFit® Basic03 medium, and cryopreservation in STEM-CELLBANKER
Miyazaki, T., Isobe, T., Nakatsuji, N., & Suemori, H. (2017).Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner. Scientific Reports, 7(1).
Cites iMatrix-511 and StemFit® Basic03 as cell culture medium
Ohta, R., Niwa, A., Taniguchi, Y., Suzuki, N., Toga, J., Yagi, E., ... & Saiko, M. (2016). Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells . Scientific Reports, 6(1).
Cites iMatrix-511 and iMatrix-411
