TLR3/ISRE Luciferase Reporter-HEK293 Cell Line

Product Code
RC1023
$10,330.00
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The TLR3/ISRE Luciferase cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 3 (TLR3) and Renilla luciferase reporter gene under the transcriptional control of the interferon stimulated response element (ISRE). Functional activity of the cell line has been validated by TLR3 ligand assay (Figure 1), in which upon activation by poly (I:C), TLR3 quickly initiates TRIF-dependent signaling pathway and mediates nuclear translocation of IRF3 that induces ISRE activity.The TLR3/ISRE activation by poly (I:C) is shown in Figure 1. 
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SKU RC1023
Size 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Application

Application:

Monitor the TLR3 signaling pathway activity.Screen for activators or inhibitors of the TLR3 signaling pathway.

Culture conditions:

Cells should be grown at 37°C with 5% CO2 using DMEM medium supplemented with 10% FBS and 1% P
Storage Immediately upon receipt, store in liquid nitrogen.
Gene id TLR3/ISRE
Shipping Temp Ambient
Supplier Name Boster Biological Technology Ltd