TLR3/IFNB Luciferase Reporter-HEK293 Cell Line

Product Code
RC1022
$10,330.00
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The TLR3/IFNB Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 3 (TLR3) and Renilla luciferase reporter gene under the transcriptional control of the human IFN-beta (IFNB) promoter. Functional activity of the cell line has been validated by TLR3 ligand assay (Figure 1), in which upon activation by poly (I:C), TLR3 quickly initiates TRIF-dependent signaling pathway and mediates nuclear translocation of IRF3 that induces IFNB promoter activity.The TLR3/IFNB activation by poly (I:C) is shown in Figure 1. 
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SKU RC1022
Size 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Application

Application:

Monitor the TLR3 signaling pathway activity.Screen for activators or inhibitors of the TLR3 signaling pathway.

Culture conditions:

Cells should be grown at 37°C with 5% CO2 using DMEM medium supplemented with 10% FBS and 1% P
Storage Immediately upon receipt, store in liquid nitrogen.
Gene id TLR3/IFNB
Shipping Temp Ambient
Supplier Name Boster Biological Technology Ltd