Spike (P.1 Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter)
Product Code
78144-1
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In Brazil, a variant called P.1 was first identified in the summer of 2020. This variant has many mutations that may lead to higher transmissibility and infectivity. The Spike (P.1) (SARS-CoV-2) Pseudotyped Lentiviruses were produced with SARS-CoV-2 Variant Spike (Genbank #QHD43416.1 with P.1 mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) as the envelope glycoproteins instead of the commonly used VSVG. These pseudovirions contain the firefly luciferase gene driven by a CMV promoter, therefore, the spike-mediated cell entry can be measured via luciferase activity. The Spike (P.1) (SARS-CoV-2) pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 (P.1) variant using a Biosafety Level 2 facility.
| SKU | 78144-1 |
|---|---|
| Size | 100 ul |
| Application | 1. Study the mechanism of viral transduction of SARS-CoV-2 (P.1) variant. 2. Screening for neutralizing antibodies for SARS-CoV-2 Spike (P.1 variant) and ACE2. |
| Storage | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle. |
| Species | SARS-CoV-2 |
| Gene synonyms | coronavirus, covid, covid-19, covid19, Sars-cov-2 P.1, B1128, sars cov 2, sarscov2, Brazil variant, Brazilian lenti, Brazil mutation, B.1.1.28 lentivirus, pseudovirus, pseudovirion, 78144-1, 78144-2 |
| Shipping Temp | -80C |
| Supplier Name | BPS |