Spike (K417T, E484K, N501Y) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter)
Product Code
AMS.78143-1
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The Spike (K417T, E484K, N501Y) (SARS-CoV-2) Pseudotyped Lentiviruses were produced with SARS-CoV-2 Variant Spike (Genbank Accession #QHD43416.1 with mutations K417T, E484K, and N501Y) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions contain the firefly luciferase gene driven by a CMV promoter, therefore, the spike-mediated cell entry can be measured via luciferase activity. The Spike (SARS-CoV-2, K417T, E484K, N501Y) pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 K417T, E484K, N501Y variant in intact cells using a Biosafety Level 2 facility.
| SKU | AMS.78143-1 |
|---|---|
| Size | 100 ul |
| Application | 1. Study the mechanism of viral transduction of SARS-CoV-2 K417T, E484K, N501Y variant. 2. Screening for neutralizing antibodies that inhibit the binding of SARS-CoV-2 Spike (K417T, E484K, N501Y variant) to ACE2. |
| Storage | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle. |
| Species | SARS-CoV-2 |
| Gene synonyms | coronavirus, covid, covid-19, covid19, Sars-cov-2 B.1.1.28.1 (P.1), sars cov 2, sarscov2, Brazil variant, Brazilian lenti, P1 lentivirus, pseudovirus, pseudovirion, 78143-1, 78143-2 |
| Shipping Temp | -80C |
| Supplier Name | BPS |