p53 Luciferase Reporter Lentivirus
Product Code
AMS.78666
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Email: [email protected]
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The p53 Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by p53 response elements located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, p53-regulated gene expression in the target cells can be monitored by measuring the luciferase activity.
Figure 1. Schematic of the lenti-vector used to generate the p53 luciferase reporter lentivirus.
| SKU | AMS.78666 |
|---|---|
| Size | 500 ul x 2 |
| Application | Screen for activators or inhibitors of p53 signaling pathwayGenerate a stable p53 luciferase reporter cell line (puromycin resistant) following puromycin selection and limiting dilution cloning |
| Storage | Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80C. |
| Shipping Temp | -80C |
| Supplier Name | BPS |