NF-?B Reporter (Luc) - HCT116 Cell Line

NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The
firefly luciferase gene is controlled by 4 copies of NF-?B response element located upstream of
the TATA promoter. Following activation by stimulants, endogenous NF-?B transcription factors
bind to the DNA response elements to induce transcription of the luciferase gene.The NF-?B-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-?B signaling
in response to stimulants such as the cytokines TNF and IL-1?, pathogen-associated
molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern
(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for
establishing cell-based screens for inhibitors that target specific NF-?B stimulating molecules.
This cell line can be further modified to allow investigation of downstream NF-?B activities as a
result of targeted genetic mutation(s).

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