MAPTrix HyGel™ - 3D Hydrogel cell culture

Use of MAPTrix^TM HyGel, which is a recombinant animal free extracellular matrix 3D hydrogel, generates a well-controlled and reproducible extracellular microenvironment for 3D cell culture.  Currently existing products, such as extracted and reconstituted basement membrane, have associated with them a diverse array of unknown cues which can be highly disadvantageous, particularly when trying to isolate the effects of specific factors. In addition, these products generally have a variable composition as well as variable mechanical properties. Lot-to-lot inconsistency of reconstituted basement membrane is another problem with many existing basement membrane products which result in significant limitations regarding their end-use.

• Free of animal derived components
• All components of MAPTrix HyGel^TM are relatively stable
• Readily able to engineer the elasticity and/or pore size of the hydrogel by adjusting the concentration of MAPTrix^TM ECM or MAPTrix^TM Linker
• Simply mixing each component can form a hydrogel within two hours, under physiological conditions
• All components are soluble in most buffer solutions
• No cytotoxicity or biocompatibility problems
• Easy to embody a mimetic microenvironment for combinatorial signaling

• 3D cell culture for primary or stem cells including HUVEC
• 3D coating for a variety of substrates such as tissue engineering scaffolds

The MAPTrix HyGel ™ matrix can be modularly engineered for designed biochemical characteristics by use of either a single MAPTrix ™ ECM product or a combination of MAPTrix™ ECM products.