MagSi Tools & MagSi Direct Coupling Kits
Magnetic tools for research & development applications
Using magnetic separation in biological applications is becoming increasingly popular. An extremely versatile technique, biomagnetic separation has a range of applications from the isolation of nucleic acids and cells, to protein purification.
Adding to our extensive collection of magnetic separation solutions are MagSi-Tools, a range of surface-activated magnetic beads as well as MagSi-Direct, a revolutionary biomolecule linkage kit.
MagSi-Tools
MagSi-Tools are surface activated magnetic particles for covalent immobilization of proteins (antibodies, enzymes), peptides, nucleic acids or other molecules of interest. The MagSi platform has a broad range of functionalization possibilities such as COOH, NH2, SH, CHO, tosyl, hydrazide, and epoxy. MagSi-Tools products are available with 600nm, 1.0 µm or 3.0 µm mean diameter. Different surface modifications and size possibilities make each bead uniquely suited to a specific application.
After coupling the molecule of interest to the magnetic particle, the resulting beads can be used in downstream applications such as:
- Preparative applications: isolating specific target proteins, antibodies, nucleic acids, cells, viruses etc
- Diagnostic applications: detecting specific target proteins, nucleic acids, cells, viruses, etc.
- Immobilizing enzymes, thereby enhancing stability and minimizing auto-catalysis. Magnetic collection of the particle/enzyme complex allows for the removal of enzyme from the reaction and its subsequent reuse in a new reaction
Intended for own development use.
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Carboxyl modified surface- for carbodiimide coupling with NH2-containing molecules.
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NH2 modified surface- for carbodiimide coupling chemistry with COOH-containing molecules or aldehyde coupling chemistry.
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Modified surface for SH coupling chemistry.
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Aldehyde modified surface- for aldehyde coupling chemistry with NH2-containing molecules.
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Tosyl modified surface- for immobilization of antibodies, glycoproteins or other aldehyde-containing molecules.
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Hydrazide modified surface- for immobilization of antibodies, glycoproteins or other aldehyde-containing molecules.
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Epoxy modified surface- for coupling to enzymes and other NH2-containing molecules.
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MagSi-Direct
Ready-to-use coupling kits
MagSi-Direct enables coupling of the biological molecule of your choice to magnetic beads. The coating of MagSi-Direct acts as a nanoglue, using electron donation from electron-rich groups of the target molecule including –COOH, -CONH, -NH2, -NHR, -NR2, -OH and –SH. As a result, MagSi-Direct allows coupling of a wide variety of medium-large biomolecules, from non-protein molecules to proteins such as antibodies, cell receptor proteins, lectins, peptide aptamers and enzymes. The coating technology has a maximum interaction with large biomolecules.
Because magnetic separation technology is gentle and does not involve harsh chemicals or conditions, MagSi-Direct opens up new avenues for isolating, purifying, or characterizing delicate cells or complexes that interact with a specific ligand.
Benefits
- Binding to beads takes only minutes
- Forms very strong, stable coordinate bonds with any electron donor on your molecule
- Binding orientation is non-specific and random. Within a population of beads, all orientations of your molecule are present and available for interactions with partners
- Much lower cost and much more flexible than biotin-streptavidin or antibody-based binding techniques
- No cloning or modifications to your molecule required
- Avoids exposing partner cells, complexes, or assays to harsh buffers or conditions
Features
| Feature | MagSi-Direct 1.0 | MagSi-Direct 3.0 |
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| Diameter of beads | 1.0 μm | 3.0 μm |
| Application | Immunoassays and capture reactions in volumes ≤ 1000 μL | Cell capture applications and capture reactions in high volumes/viscous samples |
| Components | MagSi-Direct 1.0 beads, 10X immobilization buffer, and blocking buffer | MagSi-Direct 3.0 beads, 10X immobilization buffer, and blocking buffer |
| Pack sizes available | Small (2 ml beads), Medium (10 ml beads), Large (100 ml beads) | Small (2 ml beads), Medium (10 ml beads), Large (100 ml beads) |
Magnetize almost any molecule with MagSi-Direct
- Lectins
- Ligands
- Glycans
- Haptens
- Antigens
- Enzymes
- Peptides
- Aptamers
- Cytokines
- Antibiotics
- Antibodies
- Ribozymes
- Biomarkers
- Viral particles
- Bacteriophage
- Carbohydrates
- Test compounds
- Pharmaceuticals
- Cellular receptors
- Blood components
Potential applications
There are multiple possible applications of MagSi-Direct, both in vitro and in vivo, including:
Stem Cells - Personalized Cell Therapy Research
- Use your own cell-specific ligands to isolate cells of interest
- Expose cells only to their own natural ligands, which you have "magnetized"
- Isolate or concentrate minority cell populations gently and efficiently
Protein Chemistry
- Purify delicate proteins or complexes with ease
- Simply “magnetize” any natural binding partner and use it as “bait”
- Avoid complex chromatographic separations
Flow Cytometry - FACS Cell Sorting
- Remove interfering cell populations prior to flow analysis
- Just attach your cell-specific ligand to MagSi-Direct beads and use it to pull out the interfering cells with a magnet
- Enhance FACS capabilities - add magnetic cell depletion or concentration steps before or after running a sort
Diagnostics Development
- Capture or purify ANY analyte that binds to a “magnetizable” partner that you have in hand
- Develop quantitative magnetic affinity assays
MagSi-Direct 1.0
With a mean diameter of 1.0 µm, MagSi-Direct 1.0 is especially suitable for immunoassays and capture reactions in volumes ≤ 1000µL. Includes ready-to-use MagSi-Direct 1.0 beads, 10X Immobilization Buffer and Blocking Buffer
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MagSi-Direct 3.0
With a mean diameter of 3.0 µm, MagSi-Direct is especially suitable for cell capture applications and capture reactions in high volumes or viscous samples. Includes ready-to-use MagSi-Direct 3.0 beads, 10X Immobilization Buffer and Blocking Buffer
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