HVEM/NF-?B Reporter Jurkat Recombinant Cell Line
Product Code
79310
$12,945.00
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Recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-kappaB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, CD270, GenBank Accession No. NM_003820). Following activation by human HVEM ligand LIGHT, NF-kappaB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene.Materials Required but Not Supplied: Assay Medium: Thaw Medium 2 (BPS Cat. #60184)LIGHT, His-Tag (Human) (BPS Cat. #71266LIGHT-CHO Recombinant Cell Line (BPS Cat. # 79262)96-well tissue culture-treated white clear-bottom assay plateOne-Step luciferase assay system (BPS bioscience # 60690) or other luciferase reagents for measuring firefly luciferase activityLuminometer
| SKU | 79310 |
|---|---|
| Size | 2 vials |
| Application | Screen for agonists or antagonists of LIGHT-HVEM signaling in a physiological relevant cellular context Characterize T cell-mediated immune responses of HVEM and its interactions with LIGHT Screen co-stimulatory immune checkpoint molecules |
| Storage | Store in liquid nitrogen immediately upon receipt. |
| Species | Human |
| Cell Line | JURKAT |
| Tissue Type | Lymphoid Tissue |
| Gene synonyms | HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR |
| Shipping Temp | -80C |
| Supplier Name | BPS |