HVEM/NF-?B Reporter Jurkat Recombinant Cell Line

Product Code
79310
$12,945.00
Order Support
Shipping Information
Product Updates
  • Keep up to date with product offers, resources and news

  • Sign up

Recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-kappaB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, CD270, GenBank Accession No. NM_003820). Following activation by human HVEM ligand LIGHT, NF-kappaB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene.Materials Required but Not Supplied: Assay Medium: Thaw Medium 2 (BPS Cat. #60184)LIGHT, His-Tag (Human) (BPS Cat. #71266LIGHT-CHO Recombinant Cell Line (BPS Cat. # 79262)96-well tissue culture-treated white clear-bottom assay plateOne-Step luciferase assay system (BPS bioscience # 60690) or other luciferase reagents for measuring firefly luciferase activityLuminometer
More Information
SKU 79310
Size 2 vials
Application Screen for agonists or antagonists of LIGHT-HVEM signaling in a physiological relevant cellular context Characterize T cell-mediated immune responses of HVEM and its interactions with LIGHT Screen co-stimulatory immune checkpoint molecules
Storage Store in liquid nitrogen immediately upon receipt.
Species Human
Cell Line JURKAT
Tissue Type Lymphoid Tissue
Gene synonyms HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR
Shipping Temp -80C
Supplier Name BPS