hERG (Kv11.1) - HEK293 Recombinant Cell line
Product Code
AMS.60619
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Human ERG channel has been stably expressed in HEK293 cell line and its expressionwas confirmed by Western blotting. The channel activity of hERG was characterized by a fluorescence-based assay usingthallium influx as a surrogate indicator of potassium ion channel activity coupled with athallium-sensitive fluorescent dye. When hERG-HEK293 cells were pre-loaded withthallium-sensitive dye and stimulated with stimulus buffer containing potassium/thallium,thallium ions flowed through the open hERG channels into the cells and bound the dye,generating a fluorescent signal. The hERG channel activity in hERG-HEK293 cells wasblocked by hERG channel blockers, cisapride or dofetilide, causing the fluorescentsignal triggered by thallium influx to drop to the basal level.
| SKU | AMS.60619 |
|---|---|
| Size | 2 vials |
| Storage | Immediately upon receipt, store vials in liquid nitrogen. |
| Species | Human |
| Cell Line | HEK293 |
| Tissue Type | Kidney |
| Accession No | NM_000238 |
| Gene id | NM_000238 |
| Shipping Temp | -80C |
| Supplier Name | BPS |