hERG (Kv11.1) - HEK293 Recombinant Cell line

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AMS.60619
$2,120.00
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Human ERG channel has been stably expressed in HEK293 cell line and its expressionwas confirmed by Western blotting. The channel activity of hERG was characterized by a fluorescence-based assay usingthallium influx as a surrogate indicator of potassium ion channel activity coupled with athallium-sensitive fluorescent dye. When hERG-HEK293 cells were pre-loaded withthallium-sensitive dye and stimulated with stimulus buffer containing potassium/thallium,thallium ions flowed through the open hERG channels into the cells and bound the dye,generating a fluorescent signal. The hERG channel activity in hERG-HEK293 cells wasblocked by hERG channel blockers, cisapride or dofetilide, causing the fluorescentsignal triggered by thallium influx to drop to the basal level.
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SKU AMS.60619
Size 2 vials
Storage Immediately upon receipt, store vials in liquid nitrogen.
Species Human
Cell Line HEK293
Tissue Type Kidney
Accession No NM_000238
Gene id NM_000238
Shipping Temp -80C
Supplier Name BPS