GR-GAL4 Reporter (Luc)-HEK293 Cell Line (Glucocorticoid Receptor Pathway)
Product Code
AMS.60655
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The Glucocorticoid Receptor Pathway GAL4 Reporter (Luc) - HEK293 Cell Line contains afirefly luciferase gene under the control of glucocorticoid receptor ligand binding domain that isfused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR) stably integrated intoHEK293 cells. This fusion construct activates firefly luciferase expression under the control of amultimerized GAL4 upstream activation sequence (UAS). This allows for specific detection ofglucocorticoid-induced activation of the glucocorticoid receptor without the need for individualtranscriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cellline is validated for response to stimulation of dexamethasone and to the treatment withmifepristone, an inhibitor of the glucocorticoid signaling pathway.
| SKU | AMS.60655 |
|---|---|
| Size | 2 vials |
| Application | Monitor glucocorticoid signaling pathway activity and screen activators or inhibitors of the glucocorticoid signaling pathway. |
| Storage | Immediately upon receipt, store in liquid nitrogen. |
| Species | Human |
| Cell Line | HEK293 |
| Tissue Type | Kidney |
| Gene synonyms | GAL4, Glucocorticoid Receptor |
| Shipping Temp | -80C |
| Supplier Name | BPS |