Lyophilized Exosome Standards
Our purified extracellular vesicles (EV) are obtained from a variety of biological sources: cell culture
supernatant, human plasma, serum, urine. They are purified through a
combination of tangential flow filtration (TFF) and size exclusion chromatography
(SEC). These isolated vesicles are then quantified and validated for overall
protein content, size distribution, concentration and EV specific marker expression.
ExoStd
We obtain our purified small EVs (s-EVs) from human plasma, cell culture supernatant, serum, urine and our large EVs (l-EVs) are obtained from cell conditioned media. s-EVs are purified using a combination of tangential flow filtration (TFF) and size exclusion chromatography (SEC). EVs larger than 150 nm are separated using tangential flow filtration (TFF). The isolated EVs are then quantified and validated for overall protein content, size distribution, concentration and EV specific marker expression by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Applications
- Positive control for marker assessment
- Control (spike-in) for EV quantification
- OMICS analysis
- Standardized positive controls for immunocapture performance evaluation
- Flow cytometry
- Electron microscopy
Characteristics
- High purity
- Size distribution: 50 - 120 nm
- Long term stability at 4-8° C
- Purified from biofluids collected from certified donor pools
- Purified using combination of TFF and SEC
Advantages
- Easy to reconstitute
- Easy to ship and store (+4°C)
- Long term storage stability (36 months)
- Marker characterized
- Available from a large cell line bank
Our ExoStd Process
Our ExoStd Products (Fluorescent labelling available for all standards)
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