eGFP Lentivirus (Inducible TET On)
Product Code
78629
Order Support
- Email: [email protected]
Order Support
- Email: [email protected]
Order Support
-
T: +1 (800) 987 0985 (toll free)
-
Email: [email protected]
Order Support
-
Email: [email protected]
Shipping Information
-
Delivery charges will be calculated during checkout
Product Updates
-
Keep up to date with product offers, resources and news
Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), displaying increased fluorescence and more efficient folding. Enhanced GFP Lentiviruses (Inducible TET On) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express eGFP under a tight TRE tetracycline-inducible promoter (Figure 1). After induction with Doxycycline, eGFP expression can easily be visualized and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.
Figure 1. Schematic of the lenti-vector used for the eGFP lentivirus (Inducible TET on). Note that NeoR confers resistance to Geneticin (G418).
| SKU | 78629 |
|---|---|
| Size | 500 µl x 2 |
| Application | • Ideal as a positive control for transduction with a tetracycline-inducible expression system; useful for transduction optimization.• Generation of a TET inducible stable cell line expressing eGFP with G418 selection. |
| Storage | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle. |
| Shipping Temp | -80C |
| Supplier Name | BPS |