CRISPRa (SAM) HeLa Cell Line

Product Code
78193
$2,920.00
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This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to transcriptional activator VP64. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-ÃŽB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2- tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the genomic DNA and begin transcription, inducing expression of the desired gene.
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SKU 78193
Size 2 vials
Storage Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.
Shipping Temp -80C
Supplier Name BPS