SARS-CoV-2 and COVID-19 Research Tools
Tools to advance your coronavirus research
Coronaviruses are a group of enveloped positive-stranded RNA viruses, containing four structural proteins: spike (S) glycoprotein, envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein. Spike glycoprotein is a key surface protein of coronaviruses, mediating the entrance of the virus to cells by interacting with cell surface receptor angiotensin-converting enzyme 2 (ACE2).
Six strains of coronaviruses (CoVs) that can infect humans had previously been identified until last year. HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoVHKU1 are not highly pathogenic and only cause mild respiratory diseases. SARS-CoV and MERS-CoV have caused two large-scale pandemics. Before efficient antiviral drugs or vaccines were developed for SARS-CoV or MERS-CoV, a new coronavirus was discovered in Wuhan, China.
What is SARS-CoV-2?
SARS-CoV-2, previously known as 2019 novel coronavirus/2019-nCoV, emerged in Wuhan around November 2019 causing an outbreak of disease named COVID-19. With infections spread globally, SARS-CoV-2 was declared a pandemic outbreak by the World Health Organization (WHO) on 11 March 2020. The development of vaccines, diagnostics, and therapeutics is vital for the ongoing control of the outbreak. We provide an ever-expanding collection of tools for SARS-CoV-2 and Coronavirus research, including recombinant proteins, antibodies, inhibitor screening kits, and other assay kits.
Lentivirus for SARS-CoV-2 Research
We have recently launched a set of lentiviruses for SARS-CoV-2 and COVID-19 research. We offer ACE2 lentivirus alongside SARS-CoV-2 Spike pseudotyped lentivirus, and bald lentiviral pseudovirion with additional new lentiviruses for studying cell signalling pathways. We also provide a custom lentivirus service so we can produce lentivirus fully tailored to your research needs.
SARS-CoV-2 Recombinant Proteins and Antibodies
AMSBIO offers an extensive range of high quality recombinant proteins and antibodies for tailored for SARS-CoV-2 and COVID-19 research. Our range of recombinant proteins and antibodies cover research for ACE2, N protein and Spike protein, including the RBD, S1 and S2 subunits.
Magnetic Beads and Pre-Coated Plates
Our new pre-coupled magnetic beads and antigen pre-coated plates offer a more efficient method of capturing anti-SARS-CoV-2 antibodies and ACE2 protein for biopanning, flow cytometry and a range of other applications.
SARS-CoV-2 / COVID-19 Detection Kits
We offer a series of easy-to-use, high quality kits for detection of SARS-CoV-2 and SARS-CoV-2 antibodies in a range of samples. These tools are ideal for a range of applications including epidemiology studies, SARS-CoV-2 screening for research, and determination of previous SARS-CoV-2 infection.
Reagents for SARS-CoV-2 Diagnostic Kit Development
We provide a series of well-established anti-SARS-CoV-2 antibodies, including matched antibody pairs with high sensitivity and affinity, optimized for diagnostic kit development. Alongside these antibodies, we also offer antigens designed for SARS-CoV-2 antibody detection. These are both customizable and available in bulk quantities
COVID-19 Antibody & Antigen Titer Assay Kits
This series of high quality antibody and antigen titer assay kits can be used for COVID-19 vaccine development and evaluation, as well as determining SARS-CoV-2 viral infection. The assay kits provide excellent performance, having been developed using high quality proteins with high specificity, sensitivity and reproducibility. Currently, we provide kits for IgG/M antibody detection, neutralizing antibody detection, and nucleocapsid protein antigen titer detection
Customer Spotlight: Heparan Sulfate in COVID-19 infection
HS is emerging as a key factor in infection by the SARS-CoV-2 virus. The JM403 Heparan Sulfate antibody from AMSBIO has been used in two novel assays developed and applied by Prof. Dr. Johan van der Vlag and colleagues at the Radboud University Medical Center, Nijmegen, Netherlands. The results showed that the JM403 antibody is a powerful tool to develop assays to measure plasma heparanase and HS levels in patients with COVID-19.
SARS-CoV-2 Spike Mutants
During the course of COVID-19 pandemic, several new SARS-CoV-2 variants have emerged, each with differing mutations that can have impacts on transmissibility, COVID-19 disease severity, and effectiveness of vaccines. AMSBIO now offers a series of recombinant antigens and pseudotyped lentivirus with key Spike mutations in order to evaluate the efficacy of antibodies and vaccines.
Spike (SARS-CoV-2) pseudotyped VSV delta G particles
SARS-CoV-2 Spike VSV Delta G represents a robust model to study the mechanisms of SARS-CoV-2 interaction with ACE2 (Angiotensin converting enzyme 2), to characterize neutralizing antibodies, and to screen or titrate drugs that prevent viral entry into human cells. It is the preferred pseudovirus to study SARS-CoV-2 infection of Vero-6 and Calu-3 cells.
Kits Products
We offer a series of inhibitor screening kits facilitating the identification and characterization of SARS-CoV-2 inhibitors.
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We offer a number of nucleic acid extraction kits for viruses, including the <a href="https://staging.amsbio.com/magsi-na-pathogens-kit/">MagSi-NA Pathogens Kit</a>. This kit enables easy extraction of DNA and RNA from serum, plasma, oropharyngeal swab/nasopharyngeal swab, and any other respiratory samples for pathogen detection. It utilizes magnetic beads to ensure a consistently high yield of DNA and RNA and is suitable for use with various enzymatic down-steam applications, including qPCR and qRT-PCR isothermal amplification.
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We provide a series of ready-to-use ORF clones for SARS-CoV-2 genes. These are cloned in different expression vectors with a variety of tags, with some ORF sequences codon-optimized for human cell expression and some containing native virus gene sequences. If you require an ORF cloned into a different vector or need a different viral gene sequence (mutant or fragment), contact us about our custom service.
We provide CRISPR Knock-Out Kits for both ACE2 and Transmembrane Serine Protease 2 (TMPRSS2). It has been recently shown that SARS-CoV-2 needs both ACE2 and TMPRSS2 for protein priming to enter the cell, making TMPRSS2 a potential drug target.
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We provide a number of shRNA constructs for ACE2 in a variety of vectors.
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| HOOK3 (NM_032410) Human Mass Spec Standard | 10 ug | $2455.00 | View |
| KBTBD7 (NM_032138) Human Over-expression Lysate | 20 ug | $120.00 | View |
| LSM12 (NM_152344) Human Mass Spec Standard | 10 ug | $2455.00 | View |
| Methionine Sulfoxide Reductase A (MSRA) (NM_012331) Human Mass Spec Standard | 10 ug | $2455.00 | View |
| Transient overexpression lysate of poliovirus receptor-related 1 (herpesvirus entry mediator C) (PVRL1), transcript variant 2 | 100 ug | $70.00 | View |
