Collagen-Induced Arthritis
Highly purified collagen induced arthritis
Collagen-induced arthritis (CIA) is a condition induced in mice or rats and is the most common autoimmune model used to study rheumatoid arthritis. CIA shares both immunological and pathological features with human rheumatoid arthritis, therefore it is used extensively as a model to study the pathogenesis of the disease and for testing therapeutics.
CIA can be induced in rodents and non-human primates by immunization with type II collagen emulsified with adjuvant. It has been reported that type XI collagen is also capable of inducing arthritis in mice and rats, due to the alpha 3 chain of type XI collagen being identical to the alpha 1 chain of type II collagen.
MHC Types and Species of Collagen
Susceptibility to CIA in rodents is linked to the MHC class II molecules of animals, but also depends on the species of type II collagen used for immunization. Use the table below to choose the appropriate species of type II collagen for inducing CIA in your desired rodent strain.
| Species of Type II Collagen | Mouse | Rat | Monkey | ||
|---|---|---|---|---|---|
| DBA/1 (H-2q) | B10.RIII (H-2r) | LOU (RT1q) | Lewis (RT11) | ||
| Bovine | +++ | +++ | +++ | +++ | +++ |
| Chick | +++ | - | +++ | ++ | +++ |
| Human | ++ | - | + | + | ++ |
| Porcine | +++ | +++ | +++ | +++ | Not determined |
Our CIA Products
AMSBIO supplies type II, type XI, and type IX collagen from a wide range of species to suit your research needs.
To effectively induce CIA in animals, the type of adjuvant used for emulsifying collagen is a key element. AMSBIO provides Incomplete Freund’s Adjuvant (IFA) and Complete Freund’s Adjuvant (CFA) containing various amounts of M. tuberculosis to optimize the immunization conditions for inducing CIA.
Collagen
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Incomplete and Complete Freund's Adjuvant for CIA
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Using Animal Models: Factors to Consider
The genetic background of mice, even in the same strain, will vary from vendor to vendor and can affect how the animals respond to various reagents, thus impacting the results of your experiment. Therefore, testing animals from different vendors using a defined protocol is recommended before proceeding with a large full-scale experiment.
The bacteria flora is very important to the health of the animal, including the proper functioning of the immune system. Therefore, we recommend housing animals in Specific Pathogen Free (SPF) conditions rather than conventional conditions to avoid variability caused by bacteria.
