Cas9-Expressing MDA-MB-231 cell pool

Product Code
78069
$930.00
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Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing MDA-MB-231 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
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SKU 78069
Size 2 million cells
Application 1. Quickly generating knock-out cell pools or cell lines in MDA-MB-231 cells.
2. Implementing sgRNA screens in Cas9-expressing MDA-MB-231 cells.
Storage Immediately upon receipt, store in liquid nitrogen.
Gene synonyms SpCas9 cell pool, CRISPR cell pool, Cas9 cell pool, MDA-MB-231 cells, Human metastatic mammary adenocarcinoma cells
Shipping Temp -80C
Supplier Name BPS