Cas9-Expressing HCT116 Cell line – Low expression
Product Code
AMS.78135-L
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This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool ( Bioscience, #78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.
| SKU | AMS.78135-L |
|---|---|
| Size | 2 vials |
| Application | 1. Quickly generating knock-out cell pools or cell lines in HCT116 cells. 2. Implementing sgRNA screens in Cas9-expressing HCT116 cells. |
| Storage | Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80C freezer for long term storage. |
| Tag | FLAG |
| Gene synonyms | SpCas9 cell line, CRISPR cell line, Cas9 cell line, HCT-116 human colorectal carcinoma cell line |
| Shipping Temp | -80C |
| Supplier Name | BPS |