AAV3 Luciferase-eGFP

Product Code
78463
$640.00
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Adeno-Associated Virus Serotype 3 (AAV3) shares 82% sequence homology with AAV2, and like AAV2, requires Heparan Sulfate Proteoglycan (HSPG) for cellular attachment. AAV3 vectors transduce human liver cancer cells extremely efficiently because AAV3 utilizes the human Hepatocyte Growth Factor Receptor (hHGFR) as a co-receptor for viral entry, which is highly expressed in these cells. Both the extracellular domain and the intracellular kinase domain of hHGFR are required for AAV3-mediated transgene expression. These AAV3 particles constitutively express the firefly (Photinus pyralis) luciferase and eGFP genes connected via a T2A linker, under the control of a CMV promoter. The T2A self-cleaving peptide (derived from Thosea asigna virus 2A) leads to the efficient cleavage of the transcript, and expression of luciferase and eGFP as two separate proteins.
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SKU 78463
Size 50 µl x 2
Application Use as a positive control for transductionOptimize transduction assays and track expression over time
Storage AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80C. 
Shipping Temp -80C
Supplier Name BPS